Baculovirus expression system for magnetic sorting of infected cells and enhanced titer determination

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Baculovirus expression system for magnetic sorting of infected cells and enhanced titer determination.

Recombinant baculoviruses derived from the Autographa californica nuclear polyhedrosis virus (AcNPV) are widely used to express heterologous genes in insect cells, but the use of the baculovirus expression vector system (BEVS) is hampered by slow and tedious procedures for the selection and separation of baculovirus-infected insect cells and for titer determination. Here we developed a new tech...

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cloning and expression of simian rotavirus spike protein (vp4) in insect cells by baculovirus expression system

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ژورنال

عنوان ژورنال: BioTechniques

سال: 2004

ISSN: 0736-6205,1940-9818

DOI: 10.2144/04361st03